The Division of Gaming Enforcement is a criminal justice agency as defined in s. 943.045, with the power to detect, apprehend, and arrest for any alleged violation of chapter 24, part II of chapter 285, chapter 546, chapter 550, chapter 551, or chapter 849, or any rule adopted pursuant thereto, or any law of this state. While every law enforcement officer in the state of Florida has the authority to make arrests for violations of chapter 546, chapter 550, chapter 551, or chapter 849, the Division of Gaming Enforcement is the first law enforcement agency with illegal gambling and illegal gaming as its primary responsibility.

The Division of Gaming Enforcement comprises 15 law enforcement personnel, who are divided into a North Region unit, a Central Region unit and a Southern Region unit. Each unit has a makeup of four investigators and one supervisor. In addition, the Tallahassee office of Gaming Enforcement is supported by two criminal analysts, two administrative aides, and a government operations consultant.